ev dye suspension  (Cytoskeleton Inc)

Journal: International Journal of Pharmaceutics: X

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect

doi: 10.1016/j.ijpx.2026.100487

Figure Lengend Snippet: Neutralization of merozoites. A Purification of infected erythrocytes using the Percoll separation solution, displaying infected (black arrow) and normal (red arrow) erythrocytes. B Confocal fluorescent images of merozoites (blue), PECm-Allicin@LM (red), and their colocalization (purple). Scale bar = 50 μm. C Representative scatter plots of Hoechst 33342/CFDA-SE for the invasion test of merozoites and normal erythrocytes after drug treatment. D Intrusion rate (the percentage of cells in the Q2 area in each group relative to that in the Q2 area in the Model group). Data are presented as mean ± SEM ( n = 3), * P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The red fluorescent cell membrane dye DiD was purchased from Shanghai Titan Scientific Co., Ltd. (China).

Techniques: Neutralization, Purification, Infection

Journal: Infection and Immunity

Article Title: Loss of Fgr41 in Candida albicans attenuates virulence and increases proinflammatory immune responses in a manner that is dependent on β(1,3)-glucan but not dectin-1

doi: 10.1128/iai.00523-25

Figure Lengend Snippet: Cell separation defects lead to phagocytosis of larger clumps. RAW264.7 macrophages were co-incubated with UV-inactivated C. albicans for 2 h and fixed with 4% formaldehyde. Macrophages were stained with CellBrite Green, and C. albicans cells were stained with CFW. ( A ) Confocal microscopy images of macrophages co-incubated with wild type, eng1Δ/Δ, fgr41Δ/Δ, fgr41Δ/ΔENG1 oe , or eng1Δ/Δfgr41Δ/Δ strains. Scale bar = 25 µm. ( B–D ) Quantification of panel A. A total of 40 images were analyzed for each strain. C. albicans cells were counted as being phagocytosed if they appeared internal to the macrophage and/or caused a gap in green fluorescence in the macrophage. ( B ) Percentages of macrophages containing each number of C. albicans cells in panel A. n = the total number of macrophages with at least one C. albicans cell inside them. Graphs including macrophages that had not phagocytosed any C. albicans are found in . ( C ) The number of C. albicans cells inside each macrophage for each strain from panel A. Each point represents a macrophage that had phagocytosed at least one C. albicans cell. The number of macrophages represented in each bar corresponds to the n in panel B. **** P <0.0001, *** P < 0.001, by one-way ANOVA, ns = not significant. ( D ) The wild type and eng1Δ/Δ bars from panel C were analyzed in isolation, which revealed a significant difference (* P < 0.05, by Welch’s t -test).

Article Snippet: CellBrite Green stain was purchased from Biotium (30021).

Techniques: Incubation, Staining, Confocal Microscopy, Fluorescence, Isolation